WebJan 20, 2024 · pH is a critical indicator of bone physiological function and disease status; however, noninvasive and real-time sensing of bone pH in vivo has been a challenge. Here, we synthesized a bone pH sensor by labeling alendronate with the H+-sensitive dye fluorescein isothiocyanate (Aln-FITC). Aln-FITC showed selective affinity for … WebOct 13, 2024 · The selection of the FITC form has to be considered regarding to the character of the labelling process, pH of the samples, and the solvent used in the assays. Experimental The lactone form of 5-FITC (fluorescein 5-isothiocyanate) was purchased from Sigma-Aldrich (90% purity, Germany) and the p- quinoid form from Thermo Fisher …
Measuring Phagosome pH by Ratiometric Fluorescence Microscopy
Webextremely sensitive ratiometric pH-sensitive fluorescence emis-sion. Compared to HPTS the other fluorophores like semi-naphtharhodafluor (SNARF), 2,7-bis-(2-carboxyethyl)-5-(and-6-) carboxyfluorescein (BCEF), and Fluorescein-5-isothiocyanate (FITC) are frequently used in development of various kinds of pH sensors. WebMar 15, 2024 · To enable the pH-sensitive hydrogels response toward glucose, GOx/CAT, which could convert glucose to gluconic acid, was incorporated to provide the enzyme-mediated pH stimulus. ... FITC-insulin released from the glucose responsive hydrogels IA-2 in vitro. A) Accumulated FITC-insulin released from the hydrogels incubated at different … flagstone and brick patio designs
Analysis of fluorescence lifetime and quenching of FITC ... - PubMed
WebNational Center for Biotechnology Information WebMar 10, 2016 · I am just quoting from an article " fluorescein, i.e. FITC-dextran, proved to be the most sensitive to pH, exhibiting more than a 95% decrease in fluorescence intensity as the pH decreased from 10 ... WebThe ratiometric fluorescence of FITC and PFO (F 517 /F 417) in FITC-PFO NPs solution shows an efficient pH-sensitive response over the pH range from 3 to 10 (pKa = 6.43) under two-photon excitation. Additionally, the FITC-PFO NPs is successfully applied for ratiometric imaging of intracellular pH and its fluctuation in both one-photon and two ... flagstone accent fireplaces